Composite

Part:BBa_K1039007:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-12)


Bxb1 Integrase and Recombination Directionality Factor expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 527
    Illegal XhoI site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal NgoMIV site found at 1253
    Illegal AgeI site found at 303
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1361


Design Notes

Bxb1 integrase (BBa_K1039003) has a silent mutation at base 1269, change from A to T, a silent mutation at base 273 (of gene), change G->A, still codes Gln and a silent mutation at base 1440 (of gene), change G->A, still codes Gln.


Source

Parts to create this expression cassette were synthesized by BioBasic Inc.

References